Versatile genetic assembly system (VEGAS) to assemble pathways for expression in S. cerevisiae

We have developed a method for assembling genetic pathways for expression in Saccharomyces cerevisiae. Our pathway assembly method, called VEGAS (Versatile genetic assembly system), exploits the native capacity of S. cerevisiae to perform homologous recombination and efficiently join sequences with...

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Detalles Bibliográficos
Autores principales: Mitchell, Leslie A., Chuang, James, Agmon, Neta, Khunsriraksakul, Chachrit, Phillips, Nick A., Cai, Yizhi, Truong, David M., Veerakumar, Ashan, Wang, Yuxuan, Mayorga, María, Blomquist, Paul, Sadda, Praneeth, Trueheart, Joshua, Boeke, Jef D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513848/
https://www.ncbi.nlm.nih.gov/pubmed/25956652
http://dx.doi.org/10.1093/nar/gkv466

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513848/
https://www.ncbi.nlm.nih.gov/pubmed/25956652
http://dx.doi.org/10.1093/nar/gkv466

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