New approaches for the standardization and validation of a real-time qPCR assay using TaqMan probes for quantification of yellow fever virus on clinical samples with high quality parameters

The development and production of viral vaccines, in general, involve several steps that need the monitoring of viral load throughout the entire process. Applying a 2-step quantitative reverse transcription real time PCR assay (RT-qPCR), viral load can be measured and monitored in a few hours. In th...

Descripción completa

Detalles Bibliográficos
Autores principales: Fernandes-Monteiro, Alice G, Trindade, Gisela F, Yamamura, Anna MY, Moreira, Otacilio C, de Paula, Vanessa S, Duarte, Ana Cláudia M, Britto, Constança, Lima, Sheila Maria B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514303/
https://www.ncbi.nlm.nih.gov/pubmed/26011746
http://dx.doi.org/10.4161/21645515.2014.990854

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514303/
https://www.ncbi.nlm.nih.gov/pubmed/26011746
http://dx.doi.org/10.4161/21645515.2014.990854

Ejemplares similares