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New approaches for the standardization and validation of a real-time qPCR assay using TaqMan probes for quantification of yellow fever virus on clinical samples with high quality parameters
The development and production of viral vaccines, in general, involve several steps that need the monitoring of viral load throughout the entire process. Applying a 2-step quantitative reverse transcription real time PCR assay (RT-qPCR), viral load can be measured and monitored in a few hours. In th...
Autores principales: | Fernandes-Monteiro, Alice G, Trindade, Gisela F, Yamamura, Anna MY, Moreira, Otacilio C, de Paula, Vanessa S, Duarte, Ana Cláudia M, Britto, Constança, Lima, Sheila Maria B |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514303/ https://www.ncbi.nlm.nih.gov/pubmed/26011746 http://dx.doi.org/10.4161/21645515.2014.990854 |
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