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The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model
BACKGROUND: Nearly half of all individuals with Down Syndrome (DS) have some type of congenital heart defect (CHD), suggesting that DS sensitizes to CHD but does not cause it. We used a common mouse model of DS, the Ts65Dn mouse, to study the contribution of Tbx5, a known modifier of CHD, to heart d...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514943/ https://www.ncbi.nlm.nih.gov/pubmed/26208718 http://dx.doi.org/10.1186/s12861-015-0080-y |
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author | Polk, Renita C. Gergics, Peter Steimle, Jeffrey D. Li, Huiqing Moskowitz, Ivan P. Camper, Sally A. Reeves, Roger H. |
author_facet | Polk, Renita C. Gergics, Peter Steimle, Jeffrey D. Li, Huiqing Moskowitz, Ivan P. Camper, Sally A. Reeves, Roger H. |
author_sort | Polk, Renita C. |
collection | PubMed |
description | BACKGROUND: Nearly half of all individuals with Down Syndrome (DS) have some type of congenital heart defect (CHD), suggesting that DS sensitizes to CHD but does not cause it. We used a common mouse model of DS, the Ts65Dn mouse, to study the contribution of Tbx5, a known modifier of CHD, to heart defects on a trisomic backgroun. Mice that were heterozygous for a Tbx5 null allele were crossed with Ts65Dn mice. Thoraxes of progeny were fixed in 10% formalin, embedded in paraffin, and sectioned for analysis of CHD. Gene expression in embryonic hearts was examined by quantitative PCR and in situ hybridization. A TBX5 DNA binding site was verified by luciferase assays. METHODS: Mice that were heterozygous for a Tbx5 null allele were crossed with Ts65Dn mice. Thoraxes of progeny were fixed in 10 % formalin, embedded in paraffin, and sectioned for analysis of CHD. Gene expression in embryonic hearts was examined by quantitative PCR and in situ hybridization. A TBX5 DNA binding site was verified by luciferase assays. RESULTS: We crossed mice that were heterozygous for a Tbx5 null allele with Ts65Dn mice. Mice that were trisomic and carried the Tbx5 mutation (Ts65Dn;Tbx5(+/−)) had a significantly increased incidence of overriding aorta compared to their euploid littermates. Ts65Dn;Tbx5(+/−) mice also showed reduced expression of Pitx2, a molecular marker for the left atrium. Transcript levels of the trisomic Adamts1 gene were decreased in Tbx5(+/−) mice compared to their euploid littermates. Evidence of a valid binding site for TBX5 upstream of the trisomic Adamts1 locus was also shown. CONCLUSION: Haploinsufficiency of Tbx5 and trisomy affects alignment of the aorta and this effect may stem from deviations from normal left-right patterning in the heart. We have unveiled a previously unknown interaction between the Tbx5 gene and trisomy, suggesting a connection between Tbx5 and trisomic genes important during heart development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12861-015-0080-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4514943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45149432015-07-26 The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model Polk, Renita C. Gergics, Peter Steimle, Jeffrey D. Li, Huiqing Moskowitz, Ivan P. Camper, Sally A. Reeves, Roger H. BMC Dev Biol Research Article BACKGROUND: Nearly half of all individuals with Down Syndrome (DS) have some type of congenital heart defect (CHD), suggesting that DS sensitizes to CHD but does not cause it. We used a common mouse model of DS, the Ts65Dn mouse, to study the contribution of Tbx5, a known modifier of CHD, to heart defects on a trisomic backgroun. Mice that were heterozygous for a Tbx5 null allele were crossed with Ts65Dn mice. Thoraxes of progeny were fixed in 10% formalin, embedded in paraffin, and sectioned for analysis of CHD. Gene expression in embryonic hearts was examined by quantitative PCR and in situ hybridization. A TBX5 DNA binding site was verified by luciferase assays. METHODS: Mice that were heterozygous for a Tbx5 null allele were crossed with Ts65Dn mice. Thoraxes of progeny were fixed in 10 % formalin, embedded in paraffin, and sectioned for analysis of CHD. Gene expression in embryonic hearts was examined by quantitative PCR and in situ hybridization. A TBX5 DNA binding site was verified by luciferase assays. RESULTS: We crossed mice that were heterozygous for a Tbx5 null allele with Ts65Dn mice. Mice that were trisomic and carried the Tbx5 mutation (Ts65Dn;Tbx5(+/−)) had a significantly increased incidence of overriding aorta compared to their euploid littermates. Ts65Dn;Tbx5(+/−) mice also showed reduced expression of Pitx2, a molecular marker for the left atrium. Transcript levels of the trisomic Adamts1 gene were decreased in Tbx5(+/−) mice compared to their euploid littermates. Evidence of a valid binding site for TBX5 upstream of the trisomic Adamts1 locus was also shown. CONCLUSION: Haploinsufficiency of Tbx5 and trisomy affects alignment of the aorta and this effect may stem from deviations from normal left-right patterning in the heart. We have unveiled a previously unknown interaction between the Tbx5 gene and trisomy, suggesting a connection between Tbx5 and trisomic genes important during heart development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12861-015-0080-y) contains supplementary material, which is available to authorized users. BioMed Central 2015-07-25 /pmc/articles/PMC4514943/ /pubmed/26208718 http://dx.doi.org/10.1186/s12861-015-0080-y Text en © Polk et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Polk, Renita C. Gergics, Peter Steimle, Jeffrey D. Li, Huiqing Moskowitz, Ivan P. Camper, Sally A. Reeves, Roger H. The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model |
title | The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model |
title_full | The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model |
title_fullStr | The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model |
title_full_unstemmed | The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model |
title_short | The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model |
title_sort | pattern of congenital heart defects arising from reduced tbx5 expression is altered in a down syndrome mouse model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514943/ https://www.ncbi.nlm.nih.gov/pubmed/26208718 http://dx.doi.org/10.1186/s12861-015-0080-y |
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