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Experimental validation of methods for differential gene expression analysis and sample pooling in RNA-seq

BACKGROUND: Massively parallel cDNA sequencing (RNA-seq) experiments are gradually superseding microarrays in quantitative gene expression profiling. However, many biologists are uncertain about the choice of differentially expressed gene (DEG) analysis methods and the validity of cost-saving sample...

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Detalles Bibliográficos
Autores principales: Rajkumar, Anto P., Qvist, Per, Lazarus, Ross, Lescai, Francesco, Ju, Jia, Nyegaard, Mette, Mors, Ole, Børglum, Anders D., Li, Qibin, Christensen, Jane H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515013/
https://www.ncbi.nlm.nih.gov/pubmed/26208977
http://dx.doi.org/10.1186/s12864-015-1767-y