Quantifying domain-ligand affinities and specificities by high-throughput holdup assay

Many protein interactions are mediated by small linear motifs interacting specifically with defined families of globular domains. Quantifying the specificity of a motif requires measuring and comparing its binding affinities to all its putative target domains. To this aim, we developed the high-thro...

Descripción completa

Detalles Bibliográficos
Autores principales: Vincentelli, Renaud, Luck, Katja, Poirson, Juline, Polanowska, Jolanta, Abdat, Julie, Blémont, Marilyne, Turchetto, Jeremy, Iv, François, Ricquier, Kevin, Straub, Marie-Laure, Forster, Anne, Cassonnet, Patricia, Borg, Jean-Paul, Jacob, Yves, Masson, Murielle, Nominé, Yves, Reboul, Jérôme, Wolff, Nicolas, Charbonnier, Sebastian, Travé, Gilles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521981/
https://www.ncbi.nlm.nih.gov/pubmed/26053890
http://dx.doi.org/10.1038/nmeth.3438

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521981/
https://www.ncbi.nlm.nih.gov/pubmed/26053890
http://dx.doi.org/10.1038/nmeth.3438

Ejemplares similares