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Fluorescent Protein Based FRET Pairs with Improved Dynamic Range for Fluorescence Lifetime Measurements

Fluorescence Resonance Energy Transfer (FRET) using fluorescent protein variants is widely used to study biochemical processes in living cells. FRET detection by fluorescence lifetime measurements is the most direct and robust method to measure FRET. The traditional cyan-yellow fluorescent protein b...

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Detalles Bibliográficos
Autores principales: George Abraham, Bobin, Sarkisyan, Karen S., Mishin, Alexander S., Santala, Ville, Tkachenko, Nikolai V., Karp, Matti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523203/
https://www.ncbi.nlm.nih.gov/pubmed/26237400
http://dx.doi.org/10.1371/journal.pone.0134436