CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA

Knockdown mouse models, where gene dosages can be modulated, provide valuable insights into gene function. Typically, such models are generated by embryonic stem (ES) cell-based targeted insertion, or pronuclear injection, of the knockdown expression cassette. However, these methods are associated w...

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Detalles Bibliográficos
Autores principales: Miura, Hiromi, Gurumurthy, Channabasavaiah B, Sato, Takehito, Sato, Masahiro, Ohtsuka, Masato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525291/
https://www.ncbi.nlm.nih.gov/pubmed/26242611
http://dx.doi.org/10.1038/srep12799

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525291/
https://www.ncbi.nlm.nih.gov/pubmed/26242611
http://dx.doi.org/10.1038/srep12799

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