Retention of the Native Epigenome in Purified Mammalian Chromatin

A protocol is presented for the isolation of native mammalian chromatin as fibers of 25–250 nucleosomes under conditions that preserve the natural epigenetic signature. The material is composed almost exclusively of histones and DNA and conforms to the structure expected by electron microscopy. All...

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Detalles Bibliográficos
Autores principales: Ehrensberger, Andreas H., Franchini, Don-Marc, East, Philip, George, Roger, Matthews, Nik, Maslen, Sarah L., Svejstrup, Jesper Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527833/
https://www.ncbi.nlm.nih.gov/pubmed/26248330
http://dx.doi.org/10.1371/journal.pone.0133246

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527833/
https://www.ncbi.nlm.nih.gov/pubmed/26248330
http://dx.doi.org/10.1371/journal.pone.0133246

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