Establishment and Validation of a Non-Radioactive Method for In Vitro Transcription Assay Using Primer Extension and Quantitative Real Time PCR

Primer extension-dependent in vitro transcription assay is one of the most important approaches in the research field of gene transcription. However, conventional in vitro transcription assays incorporates radioactive isotopes that cause environmental and health concerns and restricts its scope of a...

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Detalles Bibliográficos
Autores principales: Wang, Juan, Zhao, Shasha, Zhou, Ying, Wei, Yun, Deng, Wensheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529316/
https://www.ncbi.nlm.nih.gov/pubmed/26252791
http://dx.doi.org/10.1371/journal.pone.0135317

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529316/
https://www.ncbi.nlm.nih.gov/pubmed/26252791
http://dx.doi.org/10.1371/journal.pone.0135317

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