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A lipophilic fluorescent LipidGreen1-based quantification method for high-throughput screening analysis of intracellular poly-3-hydroxybutyrate

Poly-3-hydroxybutyrate (PHB), the most abundant type of polyhydroxyalkanoates (PHA) is synthesized inside a variety of microorganisms as a primary candidate for industrial PHB production. Lipophilic dyes such as Nile red and BODIPY have been used to quantify intracellular PHB, but their uses have of...

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Detalles Bibliográficos
Autores principales: Choi, Ji Eun, Na, Hye Young, Yang, Taek Ho, Rhee, Sung-Keun, Song, Jae Kwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529427/
https://www.ncbi.nlm.nih.gov/pubmed/26253390
http://dx.doi.org/10.1186/s13568-015-0131-6