Mammalian synthetic circuits with RNA binding proteins delivered by RNA

Synthetic regulatory circuits encoded on RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fa...

Descripción completa

Detalles Bibliográficos
Autores principales: Wroblewska, Liliana, Kitada, Tasuku, Endo, Kei, Siciliano, Velia, Stillo, Breanna, Saito, Hirohide, Weiss, Ron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532950/
https://www.ncbi.nlm.nih.gov/pubmed/26237515
http://dx.doi.org/10.1038/nbt.3301
_version_ 1782385278699175936
author Wroblewska, Liliana
Kitada, Tasuku
Endo, Kei
Siciliano, Velia
Stillo, Breanna
Saito, Hirohide
Weiss, Ron
author_facet Wroblewska, Liliana
Kitada, Tasuku
Endo, Kei
Siciliano, Velia
Stillo, Breanna
Saito, Hirohide
Weiss, Ron
author_sort Wroblewska, Liliana
collection PubMed
description Synthetic regulatory circuits encoded on RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded on modified mRNA or self-replicating RNA.
format Online
Article
Text
id pubmed-4532950
institution National Center for Biotechnology Information
language English
publishDate 2015
record_format MEDLINE/PubMed
spelling pubmed-45329502016-02-03 Mammalian synthetic circuits with RNA binding proteins delivered by RNA Wroblewska, Liliana Kitada, Tasuku Endo, Kei Siciliano, Velia Stillo, Breanna Saito, Hirohide Weiss, Ron Nat Biotechnol Article Synthetic regulatory circuits encoded on RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded on modified mRNA or self-replicating RNA. 2015-08-03 2015-08 /pmc/articles/PMC4532950/ /pubmed/26237515 http://dx.doi.org/10.1038/nbt.3301 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wroblewska, Liliana
Kitada, Tasuku
Endo, Kei
Siciliano, Velia
Stillo, Breanna
Saito, Hirohide
Weiss, Ron
Mammalian synthetic circuits with RNA binding proteins delivered by RNA
title Mammalian synthetic circuits with RNA binding proteins delivered by RNA
title_full Mammalian synthetic circuits with RNA binding proteins delivered by RNA
title_fullStr Mammalian synthetic circuits with RNA binding proteins delivered by RNA
title_full_unstemmed Mammalian synthetic circuits with RNA binding proteins delivered by RNA
title_short Mammalian synthetic circuits with RNA binding proteins delivered by RNA
title_sort mammalian synthetic circuits with rna binding proteins delivered by rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532950/
https://www.ncbi.nlm.nih.gov/pubmed/26237515
http://dx.doi.org/10.1038/nbt.3301
work_keys_str_mv AT wroblewskaliliana mammaliansyntheticcircuitswithrnabindingproteinsdeliveredbyrna
AT kitadatasuku mammaliansyntheticcircuitswithrnabindingproteinsdeliveredbyrna
AT endokei mammaliansyntheticcircuitswithrnabindingproteinsdeliveredbyrna
AT sicilianovelia mammaliansyntheticcircuitswithrnabindingproteinsdeliveredbyrna
AT stillobreanna mammaliansyntheticcircuitswithrnabindingproteinsdeliveredbyrna
AT saitohirohide mammaliansyntheticcircuitswithrnabindingproteinsdeliveredbyrna
AT weissron mammaliansyntheticcircuitswithrnabindingproteinsdeliveredbyrna

Ejemplares similares