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Ultrafast capillary electrophoresis isolation of DNA aptamer for the PCR amplification-based small analyte sensing

Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target. Primers were introduced at extremities of one fragment in order to generate...

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Detalles Bibliográficos
Autores principales: Fiore, Emmanuelle, Dausse, Eric, Dubouchaud, Hervé, Peyrin, Eric, Ravelet, Corinne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4533002/
https://www.ncbi.nlm.nih.gov/pubmed/26322305
http://dx.doi.org/10.3389/fchem.2015.00049