Measurement of Lipid Accumulation in Chlorella vulgaris via Flow Cytometry and Liquid-State ¹H NMR Spectroscopy for Development of an NMR-Traceable Flow Cytometry Protocol

In this study, we cultured Chlorella vulgaris cells with a range of lipid contents, induced via nitrogen starvation, and characterized them via flow cytometry, with BODIPY 505/515 as a fluorescent lipid label, and liquid-state (1)H NMR spectroscopy. In doing so, we demonstrate the utility of calibra...

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Detalles Bibliográficos
Autores principales: Bono Jr., Michael S., Garcia, Ravi D., Sri-Jayantha, Dylan V., Ahner, Beth A., Kirby, Brian J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534451/
https://www.ncbi.nlm.nih.gov/pubmed/26267664
http://dx.doi.org/10.1371/journal.pone.0134846

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534451/
https://www.ncbi.nlm.nih.gov/pubmed/26267664
http://dx.doi.org/10.1371/journal.pone.0134846

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