Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC

The contribution of the most common reciprocal translocation in childhood B-cell precursor leukemia t(12;21)(p13;q22) to leukemia development is still under debate. Direct as well as secondary indirect effects of the TEL-AML1 fusion protein are commonly recorded by using cell lines and patient sampl...

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Detalles Bibliográficos
Autores principales: Linka, Yvonne, Ginzel, Sebastian, Borkhardt, Arndt, Landgraf, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Data in Brief
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536161/
https://www.ncbi.nlm.nih.gov/pubmed/26484077
http://dx.doi.org/10.1016/j.gdata.2014.05.010
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author Linka, Yvonne
Ginzel, Sebastian
Borkhardt, Arndt
Landgraf, Pablo
author_facet Linka, Yvonne
Ginzel, Sebastian
Borkhardt, Arndt
Landgraf, Pablo
author_sort Linka, Yvonne
collection PubMed
description The contribution of the most common reciprocal translocation in childhood B-cell precursor leukemia t(12;21)(p13;q22) to leukemia development is still under debate. Direct as well as secondary indirect effects of the TEL-AML1 fusion protein are commonly recorded by using cell lines and patient samples, often bearing the TEL-AML1 fusion protein for decades. To identify direct targets of the fusion protein a short-term induction of TEL-AML1 is needed. We here describe in detail the experimental procedure, quality controls and contents of the ChIP, mRNA expression and SILAC datasets associated with the study published by Linka and colleagues in the Blood Cancer Journal [1] utilizing a short term induction of TEL-AML1 in an inducible precursor B-cell line model.
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spelling pubmed-45361612015-10-19 Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC Linka, Yvonne Ginzel, Sebastian Borkhardt, Arndt Landgraf, Pablo Genom Data Data in Brief The contribution of the most common reciprocal translocation in childhood B-cell precursor leukemia t(12;21)(p13;q22) to leukemia development is still under debate. Direct as well as secondary indirect effects of the TEL-AML1 fusion protein are commonly recorded by using cell lines and patient samples, often bearing the TEL-AML1 fusion protein for decades. To identify direct targets of the fusion protein a short-term induction of TEL-AML1 is needed. We here describe in detail the experimental procedure, quality controls and contents of the ChIP, mRNA expression and SILAC datasets associated with the study published by Linka and colleagues in the Blood Cancer Journal [1] utilizing a short term induction of TEL-AML1 in an inducible precursor B-cell line model. Elsevier 2014-05-27 /pmc/articles/PMC4536161/ /pubmed/26484077 http://dx.doi.org/10.1016/j.gdata.2014.05.010 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Data in Brief
Linka, Yvonne
Ginzel, Sebastian
Borkhardt, Arndt
Landgraf, Pablo
Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC
title Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC
title_full Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC
title_fullStr Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC
title_full_unstemmed Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC
title_short Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC
title_sort identification of tel-aml1 (etv6-runx1) associated dna and its impact on mrna and protein output using chip, mrna expression arrays and silac
topic Data in Brief
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536161/
https://www.ncbi.nlm.nih.gov/pubmed/26484077
http://dx.doi.org/10.1016/j.gdata.2014.05.010
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