Nonorthogonal tRNA(cys)(Amber) for protein and nascent chain labeling

In vitro-transcribed suppressor tRNAs are commonly used in site-specific fluorescence labeling for protein and ribosome-bound nascent chains (RNCs) studies. Here, we describe the production of nonorthogonal Bacillus subtilis tRNA(cys)(Amber) from Escherichia coli, a process that is superior to in vi...

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Detalles Bibliográficos
Autores principales: Koubek, Jiří, Chen, Yet-Ran, Cheng, Richard Ping, Huang, Joseph Jen-Tse
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2015
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536326/
https://www.ncbi.nlm.nih.gov/pubmed/26194135
http://dx.doi.org/10.1261/rna.051805.115

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536326/
https://www.ncbi.nlm.nih.gov/pubmed/26194135
http://dx.doi.org/10.1261/rna.051805.115

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