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Imaging Ca(2+) activity in mammalian cells and zebrafish with a novel red-emitting aequorin variant
Ca(2+) monitoring with aequorin is an established bioluminescence technique, whereby the photoprotein emits blue light when it binds to Ca(2+). However, aequorin’s blue emission and low quantum yield limit its application for in vivo imaging because blue-green light is greatly attenuated in animal t...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4537489/ https://www.ncbi.nlm.nih.gov/pubmed/25355614 http://dx.doi.org/10.1007/s00424-014-1639-3 |