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Site-specific labeling of RNA by combining genetic alphabet expansion transcription and copper-free click chemistry
Site-specific labeling of long-chain RNAs with desired molecular probes is an imperative technique to facilitate studies of functional RNA molecules. By genetic alphabet expansion using an artificial third base pair, called an unnatural base pair, we present a post-transcriptional modification metho...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538826/ https://www.ncbi.nlm.nih.gov/pubmed/26130718 http://dx.doi.org/10.1093/nar/gkv638 |