One Crystal, Two Temperatures: Cryocooling Penalties Alter Ligand Binding to Transient Protein Sites

Interrogating fragment libraries by X-ray crystallography is a powerful strategy for discovering allosteric ligands for protein targets. Cryocooling of crystals should theoretically increase the fraction of occupied binding sites and decrease radiation damage. However, it might also perturb protein...

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Detalles Bibliográficos
Autores principales: Fischer, Marcus, Shoichet, Brian K, Fraser, James S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2015
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4539595/
https://www.ncbi.nlm.nih.gov/pubmed/26032594
http://dx.doi.org/10.1002/cbic.201500196

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4539595/
https://www.ncbi.nlm.nih.gov/pubmed/26032594
http://dx.doi.org/10.1002/cbic.201500196

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