Cargando…

Live-cell observation of cytosolic HIV-1 assembly onset reveals RNA-interacting Gag oligomers

Assembly of the Gag polyprotein into new viral particles in infected cells is a crucial step in the retroviral replication cycle. Currently, little is known about the onset of assembly in the cytosol. In this paper, we analyzed the cytosolic HIV-1 Gag fraction in real time in live cells using advanc...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hendrix, Jelle, Baumgärtel, Viola, Schrimpf, Waldemar, Ivanchenko, Sergey, Digman, Michelle A., Gratton, Enrico, Kräusslich, Hans-Georg, Müller, Barbara, Lamb, Don C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Rockefeller University Press 2015
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4539982/ https://www.ncbi.nlm.nih.gov/pubmed/26283800 http://dx.doi.org/10.1083/jcb.201504006

Ejemplares similares

Arbitrary-Region Raster Image Correlation Spectroscopy
por: Hendrix, Jelle, et al.
Publicado: (2016)

PAM: A Framework for Integrated Analysis of Imaging, Single-Molecule, and Ensemble Fluorescence Data
por: Schrimpf, Waldemar, et al.
Publicado: (2018)

Coordinated changes of HIV-1 Gag and Env during assembly and maturation
por: Kräusslich, Hans-Georg
Publicado: (2013)

Super-Resolution Imaging of ESCRT-Proteins at HIV-1 Assembly Sites
por: Prescher, Jens, et al.
Publicado: (2015)

Dynamics of HIV-1 Assembly and Release
por: Ivanchenko, Sergey, et al.
Publicado: (2009)

Synchronized HIV assembly by tunable PIP(2) changes reveals PIP(2) requirement for stable Gag anchoring
por: Mücksch, Frauke, et al.
Publicado: (2017)

The Lateral Membrane Organization and Dynamics of Myelin Proteins PLP and MBP Are Dictated by Distinct Galactolipids and the Extracellular Matrix
por: Ozgen, Hande, et al.
Publicado: (2014)

Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly
por: Baumgärtel, Viola, et al.
Publicado: (2012)

Gauging Gag assembly
por: Short, Ben
Publicado: (2015)

Paxillin Dynamics Measured during Adhesion Assembly and Disassembly by Correlation Spectroscopy
por: Digman, Michelle A., et al.
Publicado: (2008)

Re-visiting the functional Relevance of the highly conserved Serine 40 Residue within HIV-1 p6(Gag)
por: Radestock, Benjamin, et al.
Publicado: (2014)

Protein-mediated RNA folding governs sequence-specific interactions between rotavirus genome segments
por: Borodavka, Alexander, et al.
Publicado: (2017)

Computational Identification of Novel Amino-Acid Interactions in HIV Gag via Correlated Evolution
por: Kalinina, Olga V., et al.
Publicado: (2012)

Monitoring integrity and localization of modified single-stranded RNA oligonucleotides using ultrasensitive fluorescence methods
por: Heissig, Philipp, et al.
Publicado: (2017)

High-resolution structures of HIV-1 Gag cleavage mutants determine structural switch for virus maturation
por: Mattei, Simone, et al.
Publicado: (2018)

A Loop Region in the N-Terminal Domain of Ebola Virus VP40 Is Important in Viral Assembly, Budding, and Egress
por: Adu-Gyamfi, Emmanuel, et al.
Publicado: (2014)

Gag-Pol Processing during HIV-1 Virion Maturation: A Systems Biology Approach
por: Könnyű, Balázs, et al.
Publicado: (2013)

Cell Matrix Remodeling Ability Shown by Image Spatial Correlation
por: Chiu, Chi-Li, et al.
Publicado: (2013)

Direct measurement of Gag–Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopy
por: Larson, Daniel R., et al.
Publicado: (2003)

New Insights into HTLV-1 Particle Structure, Assembly, and Gag-Gag Interactions in Living Cells
por: Fogarty, Keir H., et al.
Publicado: (2011)

Quantification of cytosolic interactions identifies Ede1 oligomers as key organizers of endocytosis
por: Boeke, Dominik, et al.
Publicado: (2014)

Spatial Characterization of Bioenergetics and Metabolism of Primordial to Preovulatory Follicles in Whole Ex Vivo Murine Ovary
por: Cinco, Rachel, et al.
Publicado: (2016)

Molecular mechanisms by which HERV-K Gag interferes with HIV-1 Gag assembly and particle infectivity
por: Monde, Kazuaki, et al.
Publicado: (2017)

New insights into retroviral Gag–Gag and Gag–membrane interactions
por: Maldonado, José O., et al.
Publicado: (2014)

Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope
por: Digman, Michelle A., et al.
Publicado: (2008)

Spatial Diffusivity and Availability of Intracellular Calmodulin
por: Sanabria, Hugo, et al.
Publicado: (2008)

Characterization of exogenous DNA mobility in live cells through fluctuation correlation spectroscopy
por: Mieruszynski, Stephen, et al.
Publicado: (2015)

Dynamics of HIV-1 Gag Processing as Revealed by Fluorescence Lifetime Imaging Microscopy and Single Virus Tracking
por: Qian, Chen, et al.
Publicado: (2022)

Actin oligomers hit the assembly line
por: Short, Ben
Publicado: (2010)

Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells
por: Andrews, Laura M., et al.
Publicado: (2012)

Monomer–dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies
por: Caiolfa, Valeria R., et al.
Publicado: (2007)

Analysis of the HTLV-1 Gag assembly pathway by biophysical fluorescence
por: Fogarty, Keir H, et al.
Publicado: (2011)

Gag Mutations Strongly Contribute to HIV-1 Resistance to Protease Inhibitors in Highly Drug-Experienced Patients besides Compensating for Fitness Loss
por: Dam, Elisabeth, et al.
Publicado: (2009)

Chemical diversity in a metal–organic framework revealed by fluorescence lifetime imaging
por: Schrimpf, Waldemar, et al.
Publicado: (2018)

Live Cell Characterization of DNA Aggregation Delivered through Lipofection
por: Mieruszynski, Stephen, et al.
Publicado: (2015)

Gag-Gag Interactions Are Insufficient to Fully Stabilize and Order the Immature HIV Gag Lattice
por: Saha, Ipsita, et al.
Publicado: (2021)

Origin of metastable oligomers and their effects on amyloid fibril self-assembly
por: Hasecke, Filip, et al.
Publicado: (2018)

Modeling the dynamics and kinetics of HIV-1 Gag during viral assembly
por: Tomasini, Michael D., et al.
Publicado: (2018)

The thermodynamics of Pr55(Gag)-RNA interaction regulate the assembly of HIV
por: Tanwar, Hanumant S., et al.
Publicado: (2017)

Early HIV-1 Gag Assembly on Lipid Membrane with vRNA
por: Zhou, Anne X.-Z., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_uqo4l4joh5ijsjl7n3unme8fqb