Assessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice

RNAi-based strategies have been used for hypomorphic analyses. However, there are technical challenges to achieve robust, reproducible knockdown effect. Here we examined the artificial microRNA (amiRNA) architectures that could provide higher knockdown efficiencies. Using transient and stable transf...

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Detalles Bibliográficos
Autores principales: Miura, Hiromi, Inoko, Hidetoshi, Tanaka, Masafumi, Nakaoka, Hirofumi, Kimura, Minoru, Gurumurthy, Channabasavaiah B., Sato, Masahiro, Ohtsuka, Masato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540464/
https://www.ncbi.nlm.nih.gov/pubmed/26285215
http://dx.doi.org/10.1371/journal.pone.0135919

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540464/
https://www.ncbi.nlm.nih.gov/pubmed/26285215
http://dx.doi.org/10.1371/journal.pone.0135919

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