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Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering
The state-of-the-art procedure for gene insertions into Trichoderma reesei is a cotransformation of two plasmids, one bearing the gene of interest and the other a marker gene. This procedure yields up to 80% transformation efficiency, but both the number of integrated copies and the loci of insertio...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Microbiology
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542242/ https://www.ncbi.nlm.nih.gov/pubmed/26150462 http://dx.doi.org/10.1128/AEM.01545-15 |