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Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries
The CRISPR-Cas9 system is a powerful tool to edit eukaryotic genomes that has recently been adapted for functional screens. Several of its applications—including the disruption of genes using Cas9-nickase and the generation of large deletions—require co-expression of two distinct guide RNAs (gRNAs)....
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Pub. Group
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4544769/ https://www.ncbi.nlm.nih.gov/pubmed/26278926 http://dx.doi.org/10.1038/ncomms9083 |