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Different Donor Cell Culture Methods Can Influence the Developmental Ability of Cloned Sheep Embryos
It was proposed that arresting nuclear donor cells in G0/G1 phase facilitates the development of embryos that are derived from somatic cell nuclear transfer (SCNT). Full confluency or serum starvation is commonly used to arrest in vitro cultured somatic cells in G0/G1 phase. However, it is controver...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546374/ https://www.ncbi.nlm.nih.gov/pubmed/26291536 http://dx.doi.org/10.1371/journal.pone.0135344 |