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Different Donor Cell Culture Methods Can Influence the Developmental Ability of Cloned Sheep Embryos

It was proposed that arresting nuclear donor cells in G0/G1 phase facilitates the development of embryos that are derived from somatic cell nuclear transfer (SCNT). Full confluency or serum starvation is commonly used to arrest in vitro cultured somatic cells in G0/G1 phase. However, it is controver...

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Detalles Bibliográficos
Autores principales: Ma, LiBing, Liu, XiYu, Wang, FengMei, He, XiaoYing, Chen, Shan, Li, WenDa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546374/
https://www.ncbi.nlm.nih.gov/pubmed/26291536
http://dx.doi.org/10.1371/journal.pone.0135344

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