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Protein Conformational Changes Are Detected and Resolved Site Specifically by Second-Harmonic Generation
We present here a straightforward, broadly applicable technique for real-time detection and measurement of protein conformational changes in solution. This method is based on tethering proteins labeled with a second-harmonic generation (SHG) active dye to supported lipid bilayers. We demonstrate our...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547196/ https://www.ncbi.nlm.nih.gov/pubmed/26287632 http://dx.doi.org/10.1016/j.bpj.2015.07.016 |