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Protein Conformational Changes Are Detected and Resolved Site Specifically by Second-Harmonic Generation

We present here a straightforward, broadly applicable technique for real-time detection and measurement of protein conformational changes in solution. This method is based on tethering proteins labeled with a second-harmonic generation (SHG) active dye to supported lipid bilayers. We demonstrate our...

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Detalles Bibliográficos
Autores principales:	Moree, Ben, Connell, Katelyn, Mortensen, Richard B., Liu, C. Tony, Benkovic, Stephen J., Salafsky, Joshua
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Biophysical Society 2015
Materias:	Proteins and Nucleic Acids
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547196/ https://www.ncbi.nlm.nih.gov/pubmed/26287632 http://dx.doi.org/10.1016/j.bpj.2015.07.016

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