Extensive mapping of an innate immune network with CRISPR

The application of the CRISPR-Cas9 system marks a major breakthrough for genetic screens, particularly in mammalian cells where high-throughput targeted gene editing has been lacking. Parnas et al (2015) apply this screening technology to mouse bone marrow-derived dendritic cells in order to study t...

Descripción completa

Detalles Bibliográficos
Autores principales: Aregger, Michael, Hart, Traver, Moffat, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2015
Materias:
News & Views
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547849/
https://www.ncbi.nlm.nih.gov/pubmed/26208794
http://dx.doi.org/10.15252/msb.20156373
Descripción
Sumario:The application of the CRISPR-Cas9 system marks a major breakthrough for genetic screens, particularly in mammalian cells where high-throughput targeted gene editing has been lacking. Parnas et al (2015) apply this screening technology to mouse bone marrow-derived dendritic cells in order to study the regulation of the immune response triggered by PAMPs. Through integrated analysis of gene knockouts in conjunction with changes in protein and mRNA expression, CRISPR screens are facilitating dissection of immune regulatory networks at unprecedented resolution.

Ejemplares similares