Extensive mapping of an innate immune network with CRISPR

The application of the CRISPR-Cas9 system marks a major breakthrough for genetic screens, particularly in mammalian cells where high-throughput targeted gene editing has been lacking. Parnas et al (2015) apply this screening technology to mouse bone marrow-derived dendritic cells in order to study t...

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Detalles Bibliográficos
Autores principales: Aregger, Michael, Hart, Traver, Moffat, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2015
Materias:
News & Views
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547849/
https://www.ncbi.nlm.nih.gov/pubmed/26208794
http://dx.doi.org/10.15252/msb.20156373
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author Aregger, Michael
Hart, Traver
Moffat, Jason
author_facet Aregger, Michael
Hart, Traver
Moffat, Jason
author_sort Aregger, Michael
collection PubMed
description The application of the CRISPR-Cas9 system marks a major breakthrough for genetic screens, particularly in mammalian cells where high-throughput targeted gene editing has been lacking. Parnas et al (2015) apply this screening technology to mouse bone marrow-derived dendritic cells in order to study the regulation of the immune response triggered by PAMPs. Through integrated analysis of gene knockouts in conjunction with changes in protein and mRNA expression, CRISPR screens are facilitating dissection of immune regulatory networks at unprecedented resolution.
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spelling pubmed-45478492015-08-28 Extensive mapping of an innate immune network with CRISPR Aregger, Michael Hart, Traver Moffat, Jason Mol Syst Biol News & Views The application of the CRISPR-Cas9 system marks a major breakthrough for genetic screens, particularly in mammalian cells where high-throughput targeted gene editing has been lacking. Parnas et al (2015) apply this screening technology to mouse bone marrow-derived dendritic cells in order to study the regulation of the immune response triggered by PAMPs. Through integrated analysis of gene knockouts in conjunction with changes in protein and mRNA expression, CRISPR screens are facilitating dissection of immune regulatory networks at unprecedented resolution. John Wiley & Sons, Ltd 2015-07-24 /pmc/articles/PMC4547849/ /pubmed/26208794 http://dx.doi.org/10.15252/msb.20156373 Text en © 2015 The Authors. Published under the terms of the CC BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle News & Views
Aregger, Michael
Hart, Traver
Moffat, Jason
Extensive mapping of an innate immune network with CRISPR
title Extensive mapping of an innate immune network with CRISPR
title_full Extensive mapping of an innate immune network with CRISPR
title_fullStr Extensive mapping of an innate immune network with CRISPR
title_full_unstemmed Extensive mapping of an innate immune network with CRISPR
title_short Extensive mapping of an innate immune network with CRISPR
title_sort extensive mapping of an innate immune network with crispr
topic News & Views
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547849/
https://www.ncbi.nlm.nih.gov/pubmed/26208794
http://dx.doi.org/10.15252/msb.20156373
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