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An equation to estimate the difference between theoretically predicted and SDS PAGE-displayed molecular weights for an acidic peptide

The molecular weight (MW) of a protein can be predicted based on its amino acids (AA) composition. However, in many cases a non-chemically modified protein shows an SDS PAGE-displayed MW larger than its predicted size. Some reports linked this fact to high content of acidic AA in the protein. Howeve...

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Detalles Bibliográficos
Autores principales: Guan, Yihong, Zhu, Qinfang, Huang, Delai, Zhao, Shuyi, Jan Lo, Li, Peng, Jinrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550835/
https://www.ncbi.nlm.nih.gov/pubmed/26311515
http://dx.doi.org/10.1038/srep13370