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Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii
Streptomyces leeuwenhoekii, isolated from the hyperarid Atacama Desert, produces the new ansamycin-like compounds chaxamycins A to D, which possess potent antibacterial activity and moderate antiproliferative activity. We report the development of genetic tools to manipulate S. leeuwenhoekii and the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551226/ https://www.ncbi.nlm.nih.gov/pubmed/26092459 http://dx.doi.org/10.1128/AEM.01039-15 |
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author | Castro, Jean Franco Razmilic, Valeria Gomez-Escribano, Juan Pablo Andrews, Barbara Asenjo, Juan A. Bibb, Mervyn J. |
author_facet | Castro, Jean Franco Razmilic, Valeria Gomez-Escribano, Juan Pablo Andrews, Barbara Asenjo, Juan A. Bibb, Mervyn J. |
author_sort | Castro, Jean Franco |
collection | PubMed |
description | Streptomyces leeuwenhoekii, isolated from the hyperarid Atacama Desert, produces the new ansamycin-like compounds chaxamycins A to D, which possess potent antibacterial activity and moderate antiproliferative activity. We report the development of genetic tools to manipulate S. leeuwenhoekii and the identification and partial characterization of the 80.2-kb chaxamycin biosynthesis gene cluster, which was achieved by both mutational analysis in the natural producer and heterologous expression in Streptomyces coelicolor A3(2) strain M1152. Restoration of chaxamycin production in a nonproducing ΔcxmK mutant (cxmK encodes 3-amino-5-hydroxybenzoic acid [AHBA] synthase) was achieved by supplementing the growth medium with AHBA, suggesting that mutasynthesis may be a viable approach for the generation of novel chaxamycin derivatives. |
format | Online Article Text |
id | pubmed-4551226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-45512262015-09-22 Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii Castro, Jean Franco Razmilic, Valeria Gomez-Escribano, Juan Pablo Andrews, Barbara Asenjo, Juan A. Bibb, Mervyn J. Appl Environ Microbiol Genetics and Molecular Biology Streptomyces leeuwenhoekii, isolated from the hyperarid Atacama Desert, produces the new ansamycin-like compounds chaxamycins A to D, which possess potent antibacterial activity and moderate antiproliferative activity. We report the development of genetic tools to manipulate S. leeuwenhoekii and the identification and partial characterization of the 80.2-kb chaxamycin biosynthesis gene cluster, which was achieved by both mutational analysis in the natural producer and heterologous expression in Streptomyces coelicolor A3(2) strain M1152. Restoration of chaxamycin production in a nonproducing ΔcxmK mutant (cxmK encodes 3-amino-5-hydroxybenzoic acid [AHBA] synthase) was achieved by supplementing the growth medium with AHBA, suggesting that mutasynthesis may be a viable approach for the generation of novel chaxamycin derivatives. American Society for Microbiology 2015-08-07 2015-09 /pmc/articles/PMC4551226/ /pubmed/26092459 http://dx.doi.org/10.1128/AEM.01039-15 Text en Copyright © 2015, Castro et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) . |
spellingShingle | Genetics and Molecular Biology Castro, Jean Franco Razmilic, Valeria Gomez-Escribano, Juan Pablo Andrews, Barbara Asenjo, Juan A. Bibb, Mervyn J. Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii |
title | Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii |
title_full | Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii |
title_fullStr | Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii |
title_full_unstemmed | Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii |
title_short | Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii |
title_sort | identification and heterologous expression of the chaxamycin biosynthesis gene cluster from streptomyces leeuwenhoekii |
topic | Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551226/ https://www.ncbi.nlm.nih.gov/pubmed/26092459 http://dx.doi.org/10.1128/AEM.01039-15 |
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