Transcription yield of fully 2′-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants

On average, mutations are deleterious to proteins. Mutations conferring new function to a protein often come at the expense of protein folding or stability, reducing overall activity. Over the years, a panel of T7 RNA polymerases have been designed or evolved to accept nucleotides with modified ribo...

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Detalles Bibliográficos
Autores principales: Meyer, Adam J., Garry, Daniel J., Hall, Bradley, Byrom, Michelle M., McDonald, Hannah G., Yang, Xu, Yin, Y. Whitney, Ellington, Andrew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551944/
https://www.ncbi.nlm.nih.gov/pubmed/26209133
http://dx.doi.org/10.1093/nar/gkv734

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551944/
https://www.ncbi.nlm.nih.gov/pubmed/26209133
http://dx.doi.org/10.1093/nar/gkv734

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