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Generation of whole genome sequences of new Cryptosporidium hominis and Cryptosporidium parvum isolates directly from stool samples

BACKGROUND: Whole genome sequencing (WGS) of Cryptosporidium spp. has previously relied on propagation of the parasite in animals to generate enough oocysts from which to extract DNA of sufficient quantity and purity for analysis. We have developed and validated a method for preparation of genomic C...

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Detalles Bibliográficos
Autores principales: Hadfield, Stephen J., Pachebat, Justin A., Swain, Martin T., Robinson, Guy, Cameron, Simon JS, Alexander, Jenna, Hegarty, Matthew J., Elwin, Kristin, Chalmers, Rachel M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552982/
https://www.ncbi.nlm.nih.gov/pubmed/26318339
http://dx.doi.org/10.1186/s12864-015-1805-9