Cargando…
Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint
Positively surface-charged poly(lactide-co-glycolide) (PLGA)/Eudragit RL nanoparticles (NPs) were designed to increase retention time and sustain release profile in joints after intra-articular injection, by forming micrometer-sized electrostatic aggregates with hyaluronic acid, an endogenous anioni...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554397/ https://www.ncbi.nlm.nih.gov/pubmed/26345227 http://dx.doi.org/10.2147/IJN.S88363 |
_version_ | 1782388047645507584 |
---|---|
author | Kim, Sung Rae Ho, Myoung Jin Lee, Eugene Lee, Joon Woo Choi, Young Wook Kang, Myung Joo |
author_facet | Kim, Sung Rae Ho, Myoung Jin Lee, Eugene Lee, Joon Woo Choi, Young Wook Kang, Myung Joo |
author_sort | Kim, Sung Rae |
collection | PubMed |
description | Positively surface-charged poly(lactide-co-glycolide) (PLGA)/Eudragit RL nanoparticles (NPs) were designed to increase retention time and sustain release profile in joints after intra-articular injection, by forming micrometer-sized electrostatic aggregates with hyaluronic acid, an endogenous anionic polysaccharide found in high amounts in synovial fluid. The cationic NPs consisting of PLGA, Eudragit RL, and polyvinyl alcohol were fabricated by solvent evaporation technique. The NPs were 170.1 nm in size, with a zeta potential of 21.3 mV in phosphate-buffered saline. Hyperspectral imaging (CytoViva(®)) revealed the formation of the micrometer-sized filamentous aggregates upon admixing, due to electrostatic interaction between NPs and the polysaccharides. NPs loaded with a fluorescent probe (1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide, DiR) displayed a significantly improved retention time in the knee joint, with over 50% preservation of the fluorescent signal 28 days after injection. When DiR solution was injected intra-articularly, the fluorescence levels rapidly decreased to 30% of the initial concentration within 3 days in mice. From these findings, we suggest that PLGA-based cationic NPs could be a promising tool for prolonged delivery of therapeutic agents in joints selectively. |
format | Online Article Text |
id | pubmed-4554397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45543972015-09-04 Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint Kim, Sung Rae Ho, Myoung Jin Lee, Eugene Lee, Joon Woo Choi, Young Wook Kang, Myung Joo Int J Nanomedicine Original Research Positively surface-charged poly(lactide-co-glycolide) (PLGA)/Eudragit RL nanoparticles (NPs) were designed to increase retention time and sustain release profile in joints after intra-articular injection, by forming micrometer-sized electrostatic aggregates with hyaluronic acid, an endogenous anionic polysaccharide found in high amounts in synovial fluid. The cationic NPs consisting of PLGA, Eudragit RL, and polyvinyl alcohol were fabricated by solvent evaporation technique. The NPs were 170.1 nm in size, with a zeta potential of 21.3 mV in phosphate-buffered saline. Hyperspectral imaging (CytoViva(®)) revealed the formation of the micrometer-sized filamentous aggregates upon admixing, due to electrostatic interaction between NPs and the polysaccharides. NPs loaded with a fluorescent probe (1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide, DiR) displayed a significantly improved retention time in the knee joint, with over 50% preservation of the fluorescent signal 28 days after injection. When DiR solution was injected intra-articularly, the fluorescence levels rapidly decreased to 30% of the initial concentration within 3 days in mice. From these findings, we suggest that PLGA-based cationic NPs could be a promising tool for prolonged delivery of therapeutic agents in joints selectively. Dove Medical Press 2015-08-24 /pmc/articles/PMC4554397/ /pubmed/26345227 http://dx.doi.org/10.2147/IJN.S88363 Text en © 2015 Kim et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Kim, Sung Rae Ho, Myoung Jin Lee, Eugene Lee, Joon Woo Choi, Young Wook Kang, Myung Joo Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint |
title | Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint |
title_full | Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint |
title_fullStr | Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint |
title_full_unstemmed | Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint |
title_short | Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint |
title_sort | cationic plga/eudragit rl nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554397/ https://www.ncbi.nlm.nih.gov/pubmed/26345227 http://dx.doi.org/10.2147/IJN.S88363 |
work_keys_str_mv | AT kimsungrae cationicplgaeudragitrlnanoparticlesforincreasingretentiontimeinsynovialcavityafterintraarticularinjectioninkneejoint AT homyoungjin cationicplgaeudragitrlnanoparticlesforincreasingretentiontimeinsynovialcavityafterintraarticularinjectioninkneejoint AT leeeugene cationicplgaeudragitrlnanoparticlesforincreasingretentiontimeinsynovialcavityafterintraarticularinjectioninkneejoint AT leejoonwoo cationicplgaeudragitrlnanoparticlesforincreasingretentiontimeinsynovialcavityafterintraarticularinjectioninkneejoint AT choiyoungwook cationicplgaeudragitrlnanoparticlesforincreasingretentiontimeinsynovialcavityafterintraarticularinjectioninkneejoint AT kangmyungjoo cationicplgaeudragitrlnanoparticlesforincreasingretentiontimeinsynovialcavityafterintraarticularinjectioninkneejoint |