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Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens

Two proteins, SghA and SghR, which were recently identified and characterized as novel bacterial virulence factors regulating the infection of plant hosts by Agrobacterium, were cloned, overexpressed and purified with high yield. Both SghA and SghR form dimers in solution. The purified SghA and SghR...

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Detalles Bibliográficos
Autores principales: Ye, Fuzhou, Wang, Chao, Fu, Qinqin, Zhang, Lian-hui, Gao, Yong-gui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555920/
https://www.ncbi.nlm.nih.gov/pubmed/26323299
http://dx.doi.org/10.1107/S2053230X15012881