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LEMming: A Linear Error Model to Normalize Parallel Quantitative Real-Time PCR (qPCR) Data as an Alternative to Reference Gene Based Methods

BACKGROUND: Gene expression analysis is an essential part of biological and medical investigations. Quantitative real-time PCR (qPCR) is characterized with excellent sensitivity, dynamic range, reproducibility and is still regarded to be the gold standard for quantifying transcripts abundance. Paral...

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Detalles Bibliográficos
Autores principales: Feuer, Ronny, Vlaic, Sebastian, Arlt, Janine, Sawodny, Oliver, Dahmen, Uta, Zanger, Ulrich M., Thomas, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556681/
https://www.ncbi.nlm.nih.gov/pubmed/26325269
http://dx.doi.org/10.1371/journal.pone.0135852