Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo

The production of high affinity, class switched antibodies produced by B cells hinges on the effective differentiation of T follicular helper (Tfh) cells. Here we define conditions specifically enhancing Tfh differentiation and providing protection in a model of influenza infection. Tfh responses we...

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Autores principales: Benson, Robert A, MacLeod, Megan KL, Hale, Benjamin G, Patakas, Agapitos, Garside, Paul, Brewer, James M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558563/
https://www.ncbi.nlm.nih.gov/pubmed/26258879
http://dx.doi.org/10.7554/eLife.06994
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author Benson, Robert A
MacLeod, Megan KL
Hale, Benjamin G
Patakas, Agapitos
Garside, Paul
Brewer, James M
author_facet Benson, Robert A
MacLeod, Megan KL
Hale, Benjamin G
Patakas, Agapitos
Garside, Paul
Brewer, James M
author_sort Benson, Robert A
collection PubMed
description The production of high affinity, class switched antibodies produced by B cells hinges on the effective differentiation of T follicular helper (Tfh) cells. Here we define conditions specifically enhancing Tfh differentiation and providing protection in a model of influenza infection. Tfh responses were associated with prolonged antigen presentation by dendritic cells (DCs), which maintained T cell/DC interactions into stage 3 (>72 hr) of activation. Blocking stage 3 interactions ablated Tfh generation, demonstrating a causal link between T cell-DC behaviour and functional outcomes. The current data therefore explain how duration of antigen presentation affects the dynamics of T cell-DC interactions and consequently determine Tfh cell differentiation in the developing immune response. DOI: http://dx.doi.org/10.7554/eLife.06994.001
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spelling pubmed-45585632015-09-08 Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo Benson, Robert A MacLeod, Megan KL Hale, Benjamin G Patakas, Agapitos Garside, Paul Brewer, James M eLife Immunology The production of high affinity, class switched antibodies produced by B cells hinges on the effective differentiation of T follicular helper (Tfh) cells. Here we define conditions specifically enhancing Tfh differentiation and providing protection in a model of influenza infection. Tfh responses were associated with prolonged antigen presentation by dendritic cells (DCs), which maintained T cell/DC interactions into stage 3 (>72 hr) of activation. Blocking stage 3 interactions ablated Tfh generation, demonstrating a causal link between T cell-DC behaviour and functional outcomes. The current data therefore explain how duration of antigen presentation affects the dynamics of T cell-DC interactions and consequently determine Tfh cell differentiation in the developing immune response. DOI: http://dx.doi.org/10.7554/eLife.06994.001 eLife Sciences Publications, Ltd 2015-08-10 /pmc/articles/PMC4558563/ /pubmed/26258879 http://dx.doi.org/10.7554/eLife.06994 Text en © 2015, Benson et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Immunology
Benson, Robert A
MacLeod, Megan KL
Hale, Benjamin G
Patakas, Agapitos
Garside, Paul
Brewer, James M
Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo
title Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo
title_full Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo
title_fullStr Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo
title_full_unstemmed Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo
title_short Antigen presentation kinetics control T cell/dendritic cell interactions and follicular helper T cell generation in vivo
title_sort antigen presentation kinetics control t cell/dendritic cell interactions and follicular helper t cell generation in vivo
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558563/
https://www.ncbi.nlm.nih.gov/pubmed/26258879
http://dx.doi.org/10.7554/eLife.06994
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