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Metabolic fingerprinting of Lactobacillus paracasei: the optimal quenching strategy
BACKGROUND: Quenching in cold buffered methanol at −40 °C has long been the preferred method for sub-second inactivation of cell metabolism during metabolic fingerprinting. However, methanol is known to cause intracellular metabolite leakage of microbial cells, making the distinction between intra-...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559878/ https://www.ncbi.nlm.nih.gov/pubmed/26337225 http://dx.doi.org/10.1186/s12934-015-0322-5 |