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Metabolic fingerprinting of Lactobacillus paracasei: the optimal quenching strategy

BACKGROUND: Quenching in cold buffered methanol at −40 °C has long been the preferred method for sub-second inactivation of cell metabolism during metabolic fingerprinting. However, methanol is known to cause intracellular metabolite leakage of microbial cells, making the distinction between intra-...

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Detalles Bibliográficos
Autores principales: Jäpelt, Kristina B., Christensen, Jan H., Villas-Bôas, Silas G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559878/
https://www.ncbi.nlm.nih.gov/pubmed/26337225
http://dx.doi.org/10.1186/s12934-015-0322-5