Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria

Current methods for producing immunoglobulin G (IgG) antibodies in engineered cells often require refolding steps or secretion across one or more biological membranes. Here, we describe a robust expression platform for biosynthesis of full-length IgG antibodies in the Escherichia coli cytoplasm. Syn...

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Detalles Bibliográficos
Autores principales: Robinson, Michael-Paul, Ke, Na, Lobstein, Julie, Peterson, Cristen, Szkodny, Alana, Mansell, Thomas J., Tuckey, Corinna, Riggs, Paul D., Colussi, Paul A., Noren, Christopher J., Taron, Christopher H., DeLisa, Matthew P., Berkmen, Mehmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560801/
https://www.ncbi.nlm.nih.gov/pubmed/26311203
http://dx.doi.org/10.1038/ncomms9072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560801/
https://www.ncbi.nlm.nih.gov/pubmed/26311203
http://dx.doi.org/10.1038/ncomms9072

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