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Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain
Hyaluronan (HA) is present in the extracellular matrix of all body tissues, including synovial fluid in joints, in which it behaves as a filter that buffers transmission of mechanical forces to nociceptor nerve endings thereby reducing pain. Using recombinant systems, mouse-cultured dorsal root gang...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560824/ https://www.ncbi.nlm.nih.gov/pubmed/26311398 http://dx.doi.org/10.1038/ncomms9095 |
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author | Caires, Rebeca Luis, Enoch Taberner, Francisco J. Fernandez-Ballester, Gregorio Ferrer-Montiel, Antonio Balazs, Endre A. Gomis, Ana Belmonte, Carlos de la Peña, Elvira |
author_facet | Caires, Rebeca Luis, Enoch Taberner, Francisco J. Fernandez-Ballester, Gregorio Ferrer-Montiel, Antonio Balazs, Endre A. Gomis, Ana Belmonte, Carlos de la Peña, Elvira |
author_sort | Caires, Rebeca |
collection | PubMed |
description | Hyaluronan (HA) is present in the extracellular matrix of all body tissues, including synovial fluid in joints, in which it behaves as a filter that buffers transmission of mechanical forces to nociceptor nerve endings thereby reducing pain. Using recombinant systems, mouse-cultured dorsal root ganglia (DRG) neurons and in vivo experiments, we found that HA also modulates polymodal transient receptor potential vanilloid subtype 1 (TRPV1) channels. HA diminishes heat, pH and capsaicin (CAP) responses, thus reducing the opening probability of the channel by stabilizing its closed state. Accordingly, in DRG neurons, HA decreases TRPV1-mediated impulse firing and channel sensitization by bradykinin. Moreover, subcutaneous HA injection in mice reduces heat and capsaicin nocifensive responses, whereas the intra-articular injection of HA in rats decreases capsaicin joint nociceptor fibres discharge. Collectively, these results indicate that extracellular HA reduces the excitability of the ubiquitous TRPV1 channel, thereby lowering impulse activity in the peripheral nociceptor endings underlying pain. |
format | Online Article Text |
id | pubmed-4560824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45608242015-09-14 Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain Caires, Rebeca Luis, Enoch Taberner, Francisco J. Fernandez-Ballester, Gregorio Ferrer-Montiel, Antonio Balazs, Endre A. Gomis, Ana Belmonte, Carlos de la Peña, Elvira Nat Commun Article Hyaluronan (HA) is present in the extracellular matrix of all body tissues, including synovial fluid in joints, in which it behaves as a filter that buffers transmission of mechanical forces to nociceptor nerve endings thereby reducing pain. Using recombinant systems, mouse-cultured dorsal root ganglia (DRG) neurons and in vivo experiments, we found that HA also modulates polymodal transient receptor potential vanilloid subtype 1 (TRPV1) channels. HA diminishes heat, pH and capsaicin (CAP) responses, thus reducing the opening probability of the channel by stabilizing its closed state. Accordingly, in DRG neurons, HA decreases TRPV1-mediated impulse firing and channel sensitization by bradykinin. Moreover, subcutaneous HA injection in mice reduces heat and capsaicin nocifensive responses, whereas the intra-articular injection of HA in rats decreases capsaicin joint nociceptor fibres discharge. Collectively, these results indicate that extracellular HA reduces the excitability of the ubiquitous TRPV1 channel, thereby lowering impulse activity in the peripheral nociceptor endings underlying pain. Nature Pub. Group 2015-08-27 /pmc/articles/PMC4560824/ /pubmed/26311398 http://dx.doi.org/10.1038/ncomms9095 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Caires, Rebeca Luis, Enoch Taberner, Francisco J. Fernandez-Ballester, Gregorio Ferrer-Montiel, Antonio Balazs, Endre A. Gomis, Ana Belmonte, Carlos de la Peña, Elvira Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain |
title | Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain |
title_full | Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain |
title_fullStr | Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain |
title_full_unstemmed | Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain |
title_short | Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain |
title_sort | hyaluronan modulates trpv1 channel opening, reducing peripheral nociceptor activity and pain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560824/ https://www.ncbi.nlm.nih.gov/pubmed/26311398 http://dx.doi.org/10.1038/ncomms9095 |
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