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A novel salt-tolerant chitobiosidase discovered by genetic screening of a metagenomic library derived from chitin-amended agricultural soil

Here, we report on the construction of a metagenomic library from a chitin-amended disease-suppressive agricultural soil and its screening for genes that encode novel chitinolytic enzymes. The library, constructed in fosmids in an Escherichia coli host, comprised 145,000 clones containing inserts of...

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Detalles Bibliográficos
Autores principales: Cretoiu, Mariana Silvia, Berini, Francesca, Kielak, Anna Maria, Marinelli, Flavia, van Elsas, Jan Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561078/
https://www.ncbi.nlm.nih.gov/pubmed/26040993
http://dx.doi.org/10.1007/s00253-015-6639-5