Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

BACKGROUND: Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages,...

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Detalles Bibliográficos
Autores principales: Capone, Simona, Horvat, Jernej, Herwig, Christoph, Spadiut, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561368/
https://www.ncbi.nlm.nih.gov/pubmed/26156850
http://dx.doi.org/10.1186/s12934-015-0292-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561368/
https://www.ncbi.nlm.nih.gov/pubmed/26156850
http://dx.doi.org/10.1186/s12934-015-0292-7

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