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Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter
BACKGROUND: Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages,...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561368/ https://www.ncbi.nlm.nih.gov/pubmed/26156850 http://dx.doi.org/10.1186/s12934-015-0292-7 |