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An optimized method for high-titer lentivirus preparations without ultracentrifugation

Lentiviral technology has proven to be a powerful tool to express exogenous genes in dividing and non-dividing cells. Currently, most protocols for generating high-titer lentivirus require ultracentrifugation, which can be an instrumental barrier for routine operations in a laboratory. In this study...

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Detalles Bibliográficos
Autores principales: Jiang, Wei, Hua, Rui, Wei, Mengping, Li, Chenhong, Qiu, Zilong, Yang, Xiaofei, Zhang, Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562269/
https://www.ncbi.nlm.nih.gov/pubmed/26348152
http://dx.doi.org/10.1038/srep13875