Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

Numerous DNA assembly technologies exist for generating plasmids for biological studies. Many procedures require complex in vitro or in vivo assembly reactions followed by plasmid propagation in recombination-impaired Escherichia coli strains such as DH5α, which are optimal for stable amplification...

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Detalles Bibliográficos
Autores principales: Kostylev, Maxim, Otwell, Anne E., Richardson, Ruth E., Suzuki, Yo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562628/
https://www.ncbi.nlm.nih.gov/pubmed/26348330
http://dx.doi.org/10.1371/journal.pone.0137466

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562628/
https://www.ncbi.nlm.nih.gov/pubmed/26348330
http://dx.doi.org/10.1371/journal.pone.0137466

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