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Synthesis, cloning and expression of a novel pre-miniproinsulin analogue gene in Escherichia coli
In the present study, a novel pre-miniproinsulin analogue was designed to have a short 9 residue sequence replacing the 35 residue C-chain, one lysine and one arginine added to the C-terminus of the B-chain in combination with glycine and arginine substitution at A21 and B29, respectively, and a 16-...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4563597/ https://www.ncbi.nlm.nih.gov/pubmed/26425357 http://dx.doi.org/10.1016/j.jare.2014.03.002 |