A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine

Herein we report the use of bromomaleimides for the construction of stable albumin conjugates via conjugation to its native, single accessible, cysteine followed by hydrolysis. Advantages over the classical maleimide approach are highlighted in terms of quantitative hydrolysis and absence of undesir...

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Autores principales: Smith, Mark E. B., Caspersen, Mikael B., Robinson, Eifion, Morais, Maurício, Maruani, Antoine, Nunes, João P. M., Nicholls, Karl, Saxton, Malcolm J., Caddick, Stephen, Baker, James R., Chudasama, Vijay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4563668/
https://www.ncbi.nlm.nih.gov/pubmed/26108475
http://dx.doi.org/10.1039/c5ob01205h
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author Smith, Mark E. B.
Caspersen, Mikael B.
Robinson, Eifion
Morais, Maurício
Maruani, Antoine
Nunes, João P. M.
Nicholls, Karl
Saxton, Malcolm J.
Caddick, Stephen
Baker, James R.
Chudasama, Vijay
author_facet Smith, Mark E. B.
Caspersen, Mikael B.
Robinson, Eifion
Morais, Maurício
Maruani, Antoine
Nunes, João P. M.
Nicholls, Karl
Saxton, Malcolm J.
Caddick, Stephen
Baker, James R.
Chudasama, Vijay
author_sort Smith, Mark E. B.
collection PubMed
description Herein we report the use of bromomaleimides for the construction of stable albumin conjugates via conjugation to its native, single accessible, cysteine followed by hydrolysis. Advantages over the classical maleimide approach are highlighted in terms of quantitative hydrolysis and absence of undesirable retro-Michael deconjugation.
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spelling pubmed-45636682015-09-29 A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine Smith, Mark E. B. Caspersen, Mikael B. Robinson, Eifion Morais, Maurício Maruani, Antoine Nunes, João P. M. Nicholls, Karl Saxton, Malcolm J. Caddick, Stephen Baker, James R. Chudasama, Vijay Org Biomol Chem Chemistry Herein we report the use of bromomaleimides for the construction of stable albumin conjugates via conjugation to its native, single accessible, cysteine followed by hydrolysis. Advantages over the classical maleimide approach are highlighted in terms of quantitative hydrolysis and absence of undesirable retro-Michael deconjugation. Royal Society of Chemistry 2015-08-07 2015-06-25 /pmc/articles/PMC4563668/ /pubmed/26108475 http://dx.doi.org/10.1039/c5ob01205h Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Smith, Mark E. B.
Caspersen, Mikael B.
Robinson, Eifion
Morais, Maurício
Maruani, Antoine
Nunes, João P. M.
Nicholls, Karl
Saxton, Malcolm J.
Caddick, Stephen
Baker, James R.
Chudasama, Vijay
A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine
title A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine
title_full A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine
title_fullStr A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine
title_full_unstemmed A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine
title_short A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine
title_sort platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4563668/
https://www.ncbi.nlm.nih.gov/pubmed/26108475
http://dx.doi.org/10.1039/c5ob01205h
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