Cargando…

Kinetic Detection of Orthogonal Protein and Chemical Coordinates in Enzyme Catalysis: Double Mutants of Soybean Lipoxygenase

[Image: see text] Soybean lipoxygenase-1 (SLO-1) is a paradigmatic enzyme system for studying the contribution of hydrogen tunneling to enzymatic proton-coupled electron transfer processes. In this study, the impact of pairs of double mutants on the properties of SLO-1 is presented. Steady-state rat...

Descripción completa

Detalles Bibliográficos
Autores principales: Sharma, Sudhir C., Klinman, Judith P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565126/
https://www.ncbi.nlm.nih.gov/pubmed/26154975
http://dx.doi.org/10.1021/acs.biochem.5b00374