A Robust and Versatile Method of Combinatorial Chemical Synthesis of Gene Libraries via Hierarchical Assembly of Partially Randomized Modules

A major challenge in gene library generation is to guarantee a large functional size and diversity that significantly increases the chances of selecting different functional protein variants. The use of trinucleotides mixtures for controlled randomization results in superior library diversity and of...

Descripción completa

Detalles Bibliográficos
Autores principales: Popova, Blagovesta, Schubert, Steffen, Bulla, Ingo, Buchwald, Daniela, Kramer, Wilfried
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565649/
https://www.ncbi.nlm.nih.gov/pubmed/26355961
http://dx.doi.org/10.1371/journal.pone.0136778

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565649/
https://www.ncbi.nlm.nih.gov/pubmed/26355961
http://dx.doi.org/10.1371/journal.pone.0136778

Ejemplares similares