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Measuring Meiotic Crossovers via Multi-Locus Genotyping of Single Pollen Grains in Barley

The detection of meiotic crossovers in crop plants currently relies on scoring DNA markers in a segregating population or cytological visualization. We investigated the feasibility of using flow-sorted haploid nuclei, Phi29 DNA polymerase-based whole-genome-amplification (WGA) and multi-locus KASP-g...

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Detalles Bibliográficos
Autores principales: Dreissig, Steven, Fuchs, Jörg, Cápal, Petr, Kettles, Nicola, Byrne, Ed, Houben, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565660/
https://www.ncbi.nlm.nih.gov/pubmed/26356084
http://dx.doi.org/10.1371/journal.pone.0137677